Limit of detection for Biobot Analytics’s E9L-NVAR orthopoxvirus assay in a wastewater context

This whitepaper focuses around the limit of detection (LoD) estimates of our E9L-NVAR singleplex qPCR assays to help our customers interpret the results from Biobot’s MPXV wastewater assay. In summary, internal calculations show that Biobot’s E9L-NVAR wastewater assay has a 95% detection probability at approximately 1.15 copies per mL of wastewater input. Additional analyses of wastewater measurements suggest that the LoD in wastewater matrices is closer to 1.9 copies/mL, similar to our estimates using a synthetic template.